A fully operational Zebrafish facility is housed in our Institute and is run by postdocs, PhD students, lab Managers and graduates students. The facility provides housing, feeding, mating and embryo procurement services for our research purpose.

In total, the fishroom includes 450 X 3.5L aquariums and 105 X 1L aquariums and hosts +/- 9000 fish.
 

We are maintaining stocks of wild type zebrafish as well as several zebrafish mutants and transgenic fishlines. Multi-fluorescent protein-expressing transgenic lines and crispr-edited lines are generated in the lab for our research.

 The lab has equipment and expertise in wholemount in-situ hybridization and immunofluoresnce, transgenesis, mRNA, morpholino injections, Crispr (KO and KI), confocal imaging.

For plasmids, morpholinos and fishes, thanks to:

  • Alexander Picker Developmental Genetics Group, Dresden, Germany
  • Leonard Zon, Howard Hughes Medical Institute,Boston, MA
  • Thomas S Becker, Developmental Neurobiology & Genomics Laboratory, University of Sydney, Australia
  • Didier Stainier, Max Planck Institute for Heart and Lung Research. Bad Nauheim. Germany
  • Julian Lewis, Cancer Research UK London Research Institute, London
  • Shannon Fisher, University of Pennsylvania, Philadelphia, PA
  • Brant M. Weinstein, Unit on Vertebrate Organogenesis, National Institutes of Health , Bethesda, MD
  • Nathan D. Lawson, University of Massachusetts Medical School, Worcester, MA
  • Chi-Bin Chien, Dept. of Neurobiology & Anatomy, University of Utah Medical Center,Salt Lake City, UT
  • Koichi Kawakami, National Institute of Genetics, Mishima-city, Shizuoka, Japan
  • Alain Ghysen, INSERM U432, Universite Montpellier II, Montpellier, France
  • Jeroen Bakkers,  Hubrecht Institute, The Netherland
  • Philip Pinheiro , Patient Lab, Oxford, UK
  • Maxine Holder, Cancer Research UK London Research Institute, London
  • Richard Mark White, Zon lab, Boston MA
  • Paolo Sordino, Stazione Zoologica Anton Dohrn, Napoli, Italia
  • Christian Mosimann, Institute of Molecular Biology, Zurich, Switzerland
  • Darren Gilmour,  EMBL Heidelberg, Germany
  • Y. Albert, Pan, Georgia Regents University, Augusta, GA, USA
  • Bernard Peers, GIGA, Liège, Belgium
  • Cheol-Hee Kim, Chungnam National University, Daejeon, Korea

And many other generous members of the zebrafish community…