Sabine Costagliola Lab

 

Developed by Sabine Costagliola ©

Zebrafish Facility



The Facility

A fully operational Zebrafish facility is housed in our Institute and is run by postdocs, PhD students, lab Managers and graduates students. The facility provides housing, feeding, mating and embryo procurement services for our research purpose.



The fishes

We are maintaining stocks of wild type zebrafish as well as several zebrafish mutants and transgenic fishlines.

Green/red fluorescent protein-expressing transgenic fishes are generated in the lab for our research.



Lab expertise

The lab has equipment and expertise in transgenesis, mRNA, morpholino injections, TALENs and wholemount in-situ hybridization.


see following publications:


Opitz R*, Maquet E*, Huisken J, Antonica F, Trubiroha A, Pottier G, Janssens V, Costagliola S. 

Transgenic zebrafish illuminate the dynamics of thyroid morphogenesis and its relationship to cardiovascular development. Dev Biol 2012: 372 (2), 203–216.


Opitz R*, Maquet E*, Zoenen M, Dadhich R, Costagliola S.

TSH receptor function is required for normal thyroid differentiation in zebrafish. Mol Endocrinol. 2011 Sep;25(9):1579-99.


Opitz.R*, M.-P. Hitz*, I. Vandernoot, A. Trubiroha, R. Abu-Khudir, M. Samuels, V. Désilets, S.Costagliola*, G. Andelfinger*, and J. Deladoëy*.

Functional zebrafish studies based on human genotyping point to netrin-1 as a link between aberrant cardiovascular development and thyroid dysgenesis.

Endocrinology. 2015: vol. 156, no. 1, pp. 377–388.


*: contributed equally.

 

For plasmids, morpholinos and fishes, thanks to:


  1. Alexander Picker Developmental Genetics Group, Dresden, Germany

  2. Leonard Zon, Howard Hughes Medical Institute,Boston, MA

  3. Thomas S Becker, Developmental Neurobiology & Genomics Laboratory, University of Sydney, Australia

  4. Didier Stainier, Max Planck Institute for Heart and Lung Research. Bad Nauheim. Germany

  5. Julian Lewis, Cancer Research UK London Research Institute, London

  6. Shannon Fisher, University of Pennsylvania, Philadelphia, PA

  7. Brant M. Weinstein, Unit on Vertebrate Organogenesis, National Institutes of Health , Bethesda, MD

  8. Nathan D. Lawson, University of Massachusetts Medical School, Worcester, MA

  9. Chi-Bin Chien, Dept. of Neurobiology & Anatomy, University of Utah Medical Center,Salt Lake City, UT

  10. Koichi Kawakami, National Institute of Genetics, Mishima-city, Shizuoka, Japan

  11. Alain Ghysen, INSERM U432, Universite Montpellier II, Montpellier, France

  12. Jeroen Bakkers,  Hubrecht Institute, The Netherland

  13. Philip Pinheiro , Patient Lab, Oxford, UK

  14. Maxine Holder, Cancer Research UK London Research Institute, London

  15. Richard Mark White, Zon lab, Boston MA

  16. Paolo Sordino, Stazione Zoologica Anton Dohrn, Napoli, Italia

  17. Christian Mosimann, Institute of Molecular Biology, Zurich, Switzerland

  18. Darren Gilmour,  EMBL Heidelberg, Germany

  19. Y. Albert, Pan, Georgia Regents University, Augusta, GA, USA

  20. Bernard Peers, GIGA, Liège, Belgium

  21. Cheol-Hee Kim, Chungnam National University, Daejeon, Korea


And many other generous members of the zebrafish   community...